|
KMID : 0880220070450040339
|
|
Journal of Microbiology
2007 Volume.45 No. 4 p.339 ~ p.343
|
|
|
Screening of Genes Related to Methylglyoxal Susceptibility
|
|
Kim In-Sook
Kim Joon-Ho
Min Bum-Chan
Lee Chang-Han
Park Chan-Kyu
|
|
|
Abstract
|
|
|
|
Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression.
|
|
|
KEYWORD
|
|
|
genome screening, methylglyoxal, dihydroxyacetone, glyoxalase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|